liaI [2019-02-01 12:37:33]

resistance against oxidative stress and cell wall antibiotics, membrane anchor for LiaH, secondary bacitracin resistance determinant

locus: BSU33130

pI: 9.30

mw: 13.24 kDa

protein length: 126 aa

gene length: 381 bp

function: resistance against oxidative stress and cell wall antibiotics

product: membrane anchor for LiaH

essential: no

synonyms: yvqI

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,398,550 → 3,398,930

The protein

[SW|Localization]

• cell membrane [Pubmed|24666271]
• forms highly dynamic membrane-associated foci under non-inducing conditions [Pubmed|24666271]
• co-localizes with [protein|AE4BF12C368468C553EED7A696D5EFC63F56CA39|LiaH] in distinct static spots at the cytoplasma membrane under stress conditions [Pubmed|24666271]

Expression and Regulation

Operons

• genes: [gene|B6D1159454969D1D578E05D5CE2259E079688510|liaI]-[gene|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH]
  description: [Pubmed|20639339]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15273097], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR]: activation, [Pubmed|16816187], in [regulon|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR regulon]

  regulation

  • induced by bacitracin and rhamnolipids, induction requires high bacitracin concentrations ([protein|search|LiaR]) [Pubmed|16816187,22092710,26815905]

• genes: [gene|B6D1159454969D1D578E05D5CE2259E079688510|liaI]-[gene|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH]-[gene|A71B0392FFF70EFFC096E7E9BD5FE30792821CDD|liaG]-[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]-[gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]-[gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]
  description: [Pubmed|14651641]

  regulation

  • ''[protein|search|liaG]'': constitutive

Biological materials

Mutant

• MGNA-B040 (yvqI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1039 NBRP B. subtilis, Japan]
• 1A980 ( ''liaI''::''erm''), [Pubmed|15273097], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A980&Search=1A980 BGSC]
• BKE33130 (Δ[gene|B6D1159454969D1D578E05D5CE2259E079688510|liaI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAGATCCTCCTTTCGT, downstream forward: _UP4_TAATATCAATATATTAGGAG
• BKK33130 (Δ[gene|B6D1159454969D1D578E05D5CE2259E079688510|liaI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAGATCCTCCTTTCGT, downstream forward: _UP4_TAATATCAATATATTAGGAG

References

Reviews

• 27344142

Original publications

• 19164152,15273097,17660417,17600057,20057163,16816187,15101989,17660417,12850135,16816187,20639339,20817675,22092710,24666271,26815905